From DNA Extraction to Phylogenetic Analysis
Thirteen comprehensive modules covering modern molecular biology techniques, from basic lab fundamentals to advanced bioinformatics analysis.
Click on any module to explore theory, protocols, and learning objectives
Introduction to molecular biology laboratory safety, equipment, pipetting techniques, and basic laboratory procedures.
Compare and contrast different DNA extraction methods: column-based vs magnetic bead extraction approaches.
Silica column-based DNA purification using Zymo Quick-DNA kit with bead-beating tissue disruption.
Modern magnetic bead DNA extraction for high molecular weight DNA preservation and automation potential.
Understanding DNA purity ratios (260/280, 260/230), concentration measurements, and quality assessment standards.
UV-Vis spectrophotometry for rapid DNA quantification and purity assessment using micro-volume samples.
Fluorescence-based DNA quantification for accurate concentration measurements using dye-binding technology.
PCR amplification of COI gene for DNA barcoding and species identification using universal primers.
Visualize and analyze PCR products using agarose gel electrophoresis and UV imaging systems.
Chain-termination DNA sequencing, chromatogram analysis, and sequence quality assessment techniques.
BLAST searches, multiple sequence alignment, and phylogenetic tree construction using IQ-TREE and MEGA.
Design and evaluate PCR primers for COI barcoding using bioinformatics tools and optimization strategies.
Enzymatic PCR cleanup using ExoCleanUp FAST to remove primers and dNTPs for high-quality Sanger sequencing.
PCR-based detection of Wolbachia endosymbionts using wsp gene amplification for disease vector biocontrol research.
The modules are designed to build on each other, creating a complete molecular biology workflow from sample collection to species identification.
Modules 0-3: Fundamentals & DNA extraction
Modules 4-6: NanoDrop & Qubit analysis
Modules 7-8: PCR & gel verification
Modules 9-12: Sequencing & phylogenetics