From Mosquito to Discovery

A Hands-On Molecular Biology Journey

Fall Quarter 2025 | Department of Entomology | UC Riverside

Explore Modules View Syllabus

Course Overview

Molecular Techniques

Master DNA extraction, PCR amplification, gel electrophoresis, and Sanger sequencing through hands-on laboratory experience.

Real-World Application

Transform mosquito specimens into species identification using COI barcoding and phylogenetic analysis.

Professional Skills

Develop laboratory techniques, data analysis abilities, and scientific communication skills essential for modern biology.

Explore the Course

Jump directly to the resources you need

Lab Modules

13 hands-on modules from DNA extraction to phylogenetic analysis

View All Modules

Theory & Protocols

Comprehensive theory content with audio narration and visual aids

View Theory

Laboratory Tools

Calculators, converters, and utilities for lab work and data collection

View Tools

Learning Outcomes

Upon successful completion of this course, students will be able to:

Extract high-quality DNA from mosquito specimens using multiple methods
Assess DNA quality and quantity using spectrophotometry and fluorometry
Design and optimize PCR primers for COI gene amplification
Perform gel electrophoresis to visualize and assess DNA quality
Analyze Sanger sequencing data and troubleshoot quality issues
Construct phylogenetic trees using modern computational methods
Identify mosquito species using DNA barcoding approaches
Document laboratory procedures following scientific standards
Present scientific findings to both technical and general audiences